No heat fixing is used in this technique so the cells remain alive on the slide.
The purpose of this exercise was to identify an unknown bacterium from a liquid culture. PEA contains an alcohol which helps denature the outer membrane of Gram negative cell walls.
Unknown stayed its green color meaning it does not use citrate as its sole carbon source. The SIM test was performed to test for sugar fermentation, indole and motility. A biopsy of mucosa is taken from the antrum of the stomach, and is placed into a medium containing urea and an indicator such as phenol red.
Bacteria are necessary for the maintenance of human health. The catalase test proved positive, Unknown rport on bacillus cereus I scroll to the next choice of positive or negative results for endospore formation.
Another limitation of the experiment was I was not able to directly autoclave my tubes the lab assistants were responsible for that task. No heat fixing is used in this technique so the cells remain alive on the slide.
The semisolid medium contains casein, amino acids, an iron-containing compound, and sulfur in the form of sodium thiosulfate. Personal photograph by author. Weakly positive organisms may take several days, and negative organisms produce no color change or yellow as a result of acid production.
The second type of illness, generally referred to as the Slow-onset Diarrheal-type, is characterized by diarrhea and abdominal pain.
More Essay Examples on Disease Rubric The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. Its role in nosocomial acquired bacteremia and wound infections in postsurgical patients has also been well defined, especially when intravascular devices such as catheters are inserted.
After comparing the data and results from the biochemical tests along with the colony morphology, shape and gram- negative unknown chart, it was clear that the unknown sample 31 was Escherichia coli.
Discussion Figure 9 a Gram positive dichotomous key used to identify Unknown Refer to Figure 9, which is the dichotomous key I used to determine my unknown bacteria. Bacillus cereus Brittany Moy Coastline Community College Abstract Bacillus cereus is a Gram-positive, facultative aerobic, spore-forming, rod-shaped bacterium that is widely distributed among the environment - Unknown Rport on Bacillus cereus Essay introduction.
The Citrate test resulted negative due to no change in color. Therefore MSA selects for salt tolerant bacteria. A small amount of the culture was transferred onto a reagent paper, and rinsed with a drop of DI water. Next, I inoculate a phenol red broth test tube with Unknown to test for carbohydrate fermentation and I also inoculate my unknown into a tryptone broth and MR-VP tube, and I streak a loopful of my unknown on a citrate slant.
Once the spores germinate, the vegetative cells can multiply and produce illness causing enterotoxins. When the spore is inside a dying bacterial cell, it is called an endospore.
EMB can inhibit the growth of gram-positive and color the colonies present due to the differential components of EMB lactose, eosin-y and methylene blue.
The sizes of the cells are more accurate with negative staining because the cells do not shrink as they do when they are heat fixed. Bacillus cereus is a Gram-positive, facultative aerobic, spore-forming, rod-shaped bacterium that is widely distributed among the environment.
Introduction The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. Unknown Lab Report Essay. The purpose of this lab is to isolate a bacterial population from the normal throat flora - Unknown Lab Report Essay introduction.
A streak plate method will be used to obtain a pure culture of a Gram positive coccus genus of bacteria. Essay about Unknown Rport on Bacillus cereus Words | 12 Pages Identifying the Unknown Bacteria: Bacillus cereus Brittany Moy Coastline Community College Abstract Bacillus cereus is a Gram-positive, facultative aerobic, spore-forming, rod-shaped bacterium that.
The first test performed on the gram positive bacteria was the Nitrate Test which turned red after adding reagents giving a positive result meaning the bacteria reduced nitrate into nitrite or something further. Browse thousands of essays from our giant database of academic papers.
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A gram stain was completed and the results were a gram positive rod, which ruled out Staphylococcus aureus, Staphylococcus epidermidis, and Enterococcus faecalis, and leaving Bacillus cereus, and Bacillus subtilis as possibilities.
A nitrate test was done next to confirm results of the gram stain.Unknown rport on bacillus cereus